This is the boat we took to meet up with NOAA Ship Ronald H Brown. Image courtesy of Walt Gurley, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS.

We started off bright and early for the second leg of the Deepwater Canyon cruise today. Our trip began on the Cape Henry Launch at ~6:00 AM in Norfolk, VA. We were taking a smaller transport to meet NOAA Ship Ronald H Brown, which was sitting at predetermined coordinates in the Atlantic. It was a grey, almost monochrome morning when we set sail, and the further we traveled the denser the fog became. Despite the dreariness, spirits were high as a new crew of researchers began what we anticipate to be a fruitful trip of collection and discovery. Along the transport we were already spotting dolphins in the near distance, hopefully a good sign of what’s to come.

We reached the ship after about a 45-minute ride in the transport boat. They picked the perfect location, for as we neared the ship the sun began peak out from behind the clouds. To board, we pulled along the right (starboard, in nautical parlance) side of the ship. Our equipment, supplies, and personal gear were first to go on. We followed after, one at a time up a rope ladder to arrive safely aboard what will be our home for the next nine days.

As we neared NOAA Ship Ronald H Brown the sun began to shine. Image courtesy of Walt Gurley, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS.

A little tired, but extremely excited, we have settled down to prepare to begin our missions. While the first leg focused on analyzing biology in and around the Deepwater Canyons, this leg will revolve around archeological research. There are countless shipwrecks along the east coast of the United States that could be of significant historical importance. Locating them, identifying them, and conducting analyses on them, however, is no easy feat. First, to locate a ship we rely on previously recorded sonar scans and recorder data to pinpoint possible locations. However, even after identifying the location of a shipwreck we do not necessarily know if it is the exact ship we are looking for until further research is completed. Finally, to study the ship we use the video, still-image, and mechanical capabilities of the ROV Jason to capture footage and collect samples. So, after much research and careful planning we will begin our search for several early twentieth century shipwrecks.

Our gear was first placed on the ship. Then each person donned a life vest and climbed up the rope ladder. Image courtesy of Walt Gurley, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS.

Do not fret though biophiles; shipwrecks also form a superb marine habitat in addition to being historically significant, and we expect to see an abundance of life that inhabits the areas in and around the wreck. There will be even more biological sampling performed at night in locations not related to the wrecks themselves as well as bottom mapping and depth profiling using a CTD sensor.

Dr. Katherine Coykendall takes a genetics sample in the lab. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

19 Science team members

12 ROV dives

492 two-hour DVDs of dive footage recorded

10 ROV crew

367 genetics samples

335 gallons of seawater collected

1,660 isotope samples

1,009.5 nautical miles covered

30 ship’s crew

12 plastic site markers made

100 pounds of bacon eaten

32 box cores

43,000 gallons of fuel burned

30 CTDs

34 Push cores

Dr. Nancy Prouty drains water from the Niskin bottle and will use it to help understand the water column. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

2,250 eggs consumed

20 Mono cores

1,068 pounds of mud collected

2 landers recovered and redeployed

60,000 gallons of freshwater made and used

Bags of mud stored in the freezer for later analysis. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

1 music video

14 trawls

1,941 specimen photos

20 degree difference in air temperature between the lab and the ROV control van

294 octocoral samples collected

50+ live animals collected and maintained

2 shrunken wig heads

The manipulator arm of the Jason collecting mussels at the seep site.
Image courtesy of Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

Bathymodiolin mussels, all in the genus Bathymodiolus, evolved in environments very different than the ones we land-dwelling organisms, that get our energy indirectly from the sun through plants, did.  Bathymodiolus species live in areas of the sea floor where fluids of chemicals, such as methane and sulfide gasses, are escaping through the sea floor.  Not only can these mussels tolerate the chemicals that would be toxic to most marine organisms, they thrive here! They can filter feed, like many sedentary marine organisms (including intertidal mussels) but they also have unique microbes that live in their gills as symbionts. The symbiotic microbes can convert methane and/or sulfide to energy (chemosynthesis), and pass it along to their mussel hosts.  It is such an efficient relationship that the mussels get most of their nutrition from the microbes, and have a reduced digestive tract.

Animals that have chemosynthetic bacterial symbionts acquire them in one of two ways: vertically or horizontally.  Vertical transmission means that adult host animals pass their symbionts to their offspring. Like a favorite piece of jewelry handed down through generations, the symbiont is transferred to the young mussel.   Some species of deep sea clams acquire their symbionts this way.  Horizontal transmission means that at some point early in their development, host organisms capture symbionts from free-living bacteria in the environment. This would be more akin to going and buying a new piece of jewelry. Studies have shown that Bathymodiolus mussels acquire its symbionts this way.

Scientists can determine which mode of transmission a host uses by looking at the DNA of the host and the DNA of the chemosynthetic bacteria that live inside them.  If vertical transmission is the norm, then one would expect that the pattern of evolution of the chemosynthetic bacteria to mirror that of the host’s.  The closest relatives of the bacteria would be found in the closest relatives of the hosts. After many generations of being housed within the host, bacteria tend to lose some functional genes as they rely more and more on the host, so they have a reduced genome.

Dr. Steve Ross watches the feed from Jason which shows and expansive mussel bed. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

On the other hand, in horizontal transmission, every new generation of host captures a sample of bacteria from the environment.  The evolutionary history of the bacteria and the host would not be parallel.  Also, because the bacteria have to survive out in the environment and as a symbiont within a host, it has to have a complete, fully functioning genome.  Interestingly, some species of Bathymodiolus have just methanotrophic symbionts, while others host both methanotrophs and thiotrophs (sulfide utilizers).

These mussels are often the first species to arrive at a new gas seep site and can cover large areas of the sea floor, as we saw on our dives.  Finding a vast mussel bed without other characteristic seep animals like tubeworms may indicate that the seep is relatively young.  The mussels may also actively exclude other potential settlers to the seep site by feeding on their larvae.

Even dead shells help us understand the seep ecosystem.
Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

There are 35 species of Bathymodiolus mussels described to date, and 9 of these occur in the Atlantic, with the majority (7) found at cold seep sites. Similar shell morphology (shape) and soft tissue anatomy means that traditional taxonomy based on appearance has proven difficult as the sole means to distinguish among species.

We can delineate species by sequencing a section of DNA and comparing these sequences among species. From such analyses, two major groupings within the Bathymodiolus genus have been detected:  The B. childressi group that includes some Pacific species and tends to be at shallower sites; and the B. boomerang/heckerae group, with both hydrothermal vent and cold seep species and tends to be found at greater depth. B. heckerae is found at Blake Ridge, a cold seep off of the Carolinas, as well as a seep off the west coast of Florida. We found bathymodiolin mussels at cold seeps in both Norfolk  and Baltimore Canyons. By sequencing a barcoding gene (COX1) from several mussels from Baltimore Canyon collected last year, we know that the mussels from that seep site are B. childressi, a species that has one type of symbiont that oxidizes methane. We have been discussing which species of mussels we found – childressi, boomerang, or heckerae, or perhaps a combination! Once we get back to the lab, we will sequence this same stretch of DNA from our samples from the deeper seep off of Cape Henry,Virginia and get a molecular identification of this species.

Mussels lined up for a specimen photo which will be part of the scientific data kept about each sample. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

Although the genus Bathymodiolus was described only 27 years ago, many new species of Bathymodiolus mussels are being described from hydrothermal vent and chemosynthetic communities. Our sampling will help build a richer understanding of these unique bivalves.

Common dolphins running alongside the ship during the lander recovery. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

Although our primary purpose out here is studying what lives in the deepwater canyons, we can’t help noticing the life on the surface of the sea. It is not unusual to spot whale blows in the distance, or to have a call from the bridge “dolphins on the starboard fantail” which draws us all out of the lab.

A common tern on the A-Frame. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

Birds are common. It is not unusual for us to see gulls circling (and occasionally landing on) the ship. Terns are frequent visitors too.  During the Jason recovery a common tern, Sterna hirundo, first settled on the A-frame (that holds the Medea) and then on the LARS (that holds the Jason).  This bird breeds from Canada to the Carolinas, as well as southern Scandinavia, and winters in the Caribbean, southern Florida and northern Africa.  As its name suggests, it is quite common!

A Wilson’s storm petrel, about to take off after being found on the ship. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

Another frequent sighting is the Wilson’s storm petrel. This small black bird has a distinctive white patch on its rump, and appears to “walk on water” as it dabbles its webbed feet on the surface. It nests in Antarctica but spends the rest of its life at sea. One got trapped in some water on the deck of the ship. After extracting it, and letting it get warm and dry, we released it, and it flew off over the waves.

An osprey flying overhead during the recovery of Jason. Image courtesy of Liz Baird, Deepwater Canyons 2013, NOAA-OER/BOEM/USGS

During the recovery of Jason an osprey, Pandion haliaetus, flew by. This was unusual because ospreys tend to stay within 10 km of their roosting site. We wondered if perhaps the bird we saw was migratory. Ospreys breed on every continent except Antarctica so perhaps this one was returning north after spending the winter in South America. Ospreys are skilled hunters with an outer toe that is reversible, which allows them to carry fish with two toes on each side.  Their species name, haliaetus, comes from the Greek “halos” which means sea, and “aetus” which means eagle; a fitting name for a bird with hunting abilities that were so amazing there was a Medieval belief that the osprey mesmerized the fish, making them turn belly up.

We have had several calls for seeing a “fin” in the water. On two occasions it was a Mola mola or ocean sunfish. These unusually shaped fish appear to be “all head” and are as tall as they are long. They have a long dorsal fin on the top and long anal fin on the bottom, and not much of a body.  This unusual shape has led to a variety of names. Mola means millstone in Latin, in reference to its round shape. Most European names mean “moonfish” in reference to the shape, however its English name, “sunfish,” comes from its habit of sunbathing at the surface. It propels itself through the water by a sculling motion with its fins.  It is the heaviest bony fish known — an average adult can weigh 2,200 pounds. Females produce more eggs (300 million!) than any other known vertebrate. The young fish (called fry) are spiky and resemble miniature pufferfish. They eat jellyfish, squid, salps, and crustaceans found throughout the water column.  The Museum has a replica of one on display on the second floor of the Main Building if you want to check one out!

We watched this shark swim off the stern. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

We have also seen other fins in the water, including dolphins. We saw common dolphins playing in the waves and they put on quite a show. We have seen fins that we are certain belong to whales. Early in the cruise we spotted a minke whale. We could see the blow, a fin, and a bit of its back as it dove. During our weekly safety drill we spotted a blow and a fluke in the distance as we stood outside with our lifejackets on. We decided that had to be a humpback. We have spotted other blows in the distance (last night there were 8 distinct blows in a 2 minute span!) but have not been able to see a fin. Two pairs of shark fins were spotted one afternoon, and we could catches glimpses of the fins between the swells. We could not decide what type of shark they were and could not even agree on how big they were! Our length estimates ranged from the conservative of 8 feet to the high end of 20 feet.

The majority of our day is spent indoors, working in the science labs or the ROV Control Van, but when we get outside we look for the wildlife of the surface.

A common tern flying alongside the R/V Ron Brown. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

The lander bobbed to the surface just off the bow of the R/V Ron Brown. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOE/USGS

It is always traumatic to deploy very expensive science gear into the marine environment.  Like fishermen, the military, or anyone who uses the ocean, sometimes gear is lost.  For us, this risk is even more troublesome because in addition to perhaps losing equipment worth many thousands of dollars, we also lose invaluable data and experiments.  But, if we do not take these risks, we will learn nothing, and the quest for knowledge that will help us understand and manage our ecosystems is of vital importance.

—Steve W. Ross and Sandra Brooke

Everyone was all smiles when we found the lander.
Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

This morning we successfully recovered the first UNCW lander  deployed last fall. Imagine a triangular bunk bed outfitted with a variety of instruments.  There are probes for monitoring water chemistry, such as dissolved oxygen. A wide variety of settling plates of different materials hang on the structure. Some made of of steel, some of limestone, some of sandstone for examining microbial growth. There were other plates made from plastic foam and mesh for determining if texture plays a role in settling. A rotating sediment cup trap, which collects samples for 30 day periods before switching to a new cup, is on it, as well as a current meter called an ADCP (Acoustic Doppler Current Profiler). This data allows us to do some interesting things, such as matching the sediment by month with the current data for that month. That might allow us to say “this flow brings organic matter,” or “this flow has sandy sediment,” etc. We will be able to tell when the current reverses and can characterize water masses in the canyons by looking at things such as speed, direction, temperature and salinity.  This long-term data will give us a more complete picture of what is happening over time, which will complement the “snapshot” we get with an ROV dive.

Mike Gray and Kirstin Meyer looking at the experiments that have come up on the lander. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

Soon after the lander hit the bottom last year, the ship triangulated its position. This gave us an approximate position of the lander to go by this morning. We hovered over its calculated position and Mike Rhode (UNCW) signaled its acoustic releases to drop the 600 pound weight that anchored the lander to the seafloor.  By constantly pinging the lander for its range, he could tell it was rising off the bottom.  Based on its rate of ascent, we estimated it would get to the surface at 7:10.

These eggs are one of the things found attached to the lander. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

Everyone was scanning the horizon, and right on time, the lander surfaced just off the bow of the R/V Ronald H. Brown. We could see the red flag and yellow floats as well as the trailing float line. Several Common Dolphins were spotted nearby, we suspect attracted to the pinging of the lander. The ship steamed alongside the lander, grappling its recovery line, and used a crane to lift the lander on board. The sea swells this morning made the lander swing as we brought it on deck, which was challenging. However the ship’s crew and science team got it on board safely.  After being tied down, the lander was surrounded by scientists taking pictures, examining their experiments, and getting it ready to redeploy later today.

Here is the wig head created by Bruce Cowden based on your suggestions. She is a version of Mother Earth with the globe on the back of her head and images of ships and whales (inspired by scrimshaw). We hope to send her down tomorrow!

One of the pycnogonids collected in the trawl.
Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

A question from Zach came through the blog recently, asking about the coolest animal we have brought up so far. Pycnogonids, or sea spiders, was the answer, and after learning more about them, an entire post on these extraordinary creatures was due.

These “sea spiders” are found all over the world, from shallow water reefs in the tropics to these deep areas of the canyons. They range in size from about a third of an inch to 35 inches.  Although they are arthropods, they aren’t truly spiders, and there is debate where they should be classified. There is some thought that they are an ancient sister group to all living arthropods.  Pycnogonid means “thick knees” and they certainly have obvious joints along their legs. When they walk, the resemblance to spiders is unmistakable, however they can also swim by pulsing their legs to propel themselves through the water.

The dark orange proboscis is nearly as long as the yellow thorax of this pycnogonid. The ovigers are bent backwards towards the head, looking like paired loops in this image. Looking closely, you might see the hooks at the end of the ovigers.
Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER?BOEM/USGS

Holding one in your hand it is difficult to tell which side is up. The super skinny body and long proboscis (for feeding) don’t provide many clues. However if you look closely you can find two tiny eyes, which indicate the top of the head. The proboscis is used for feeding. The pycnogonid inserts it into animals such as anemones and sucks nutrients out,  much like a mosquito sucks blood from humans. The anemone does not die, just as we do not die from a mosquito bite. The proboscis on the specimens we collected is nearly as long at the entire body!

The eyes are located where the dark orange proboscis joins the head. On either side of the proboscis are the chelifores, for feeding and the next appendage out are the ovigers. The yellow legs join the very thin thorax.
Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS.

The anatomy of the pycnogonid is very confusing. Most pycnogonids have 4 pairs of walking legs, as do spiders. However there are some species with 5 or 6 pairs of walking legs. Most of us have seen the diagram showing three body parts for many arthropods  – head, thorax and abdomen. In pycnogonids the thorax, where the legs attach, is the main “body” and the abdomen is tiny and almost vestigial. This means there is not much room for organs inside! There are several adaptations to this, for example pycnogonids do not have a respiratory system, rather they “breathe” through diffusion. Their long skinny legs and skinny body provide lots of surface area for this process. Their digestive tract has pockets that stretch down into their legs. They have very high blood pressure, with a heart rate between 90 and 180 beats per minute. Like spiders, they have some additional appendages near their head. One pair is called an “oviger.” The pycnogonid females use this to hand eggs to the males. The males use the ovigers during courtship and to guard the eggs during development. They have pairs of chelifores and palps which are similar to chelicerae and pedipalps found in spiders and are used for feeding and sensing the environment.

Pycnogonids were found in trawling operations during the Challenger Expedition between 1873-1876. I can imagine the confusion these creatures must have caused when they came up in the net. During that voyage they collected more than 4,000 previously unknown species and laid the groundwork for the science of oceanography. And here we are, 130 years later, still asking questions about these bizarre looking creatures.

All of us out here on the R/V Ron Brown want to say “Happy Mother’s Day!”

from the science team, the ship’s crew and the Jason crew

Thank you!

Science team and crew members get ready to deploy the trawl. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER?BOEM/USGS

On this cruise the ROV Jason II is our main science sampling tool; however, underwater vehicles cannot stay on the bottom indefinitely nor can they collect every type of sample needed.  We use a variety of gear specific to different needs, and many types of nets are included in our arsenal of sampling gear.  On this cruise we are using a bottom otter trawl to collect fishes and invertebrates.  This is a small net (16 ft wide) that is held open by water pressure on otter doors (rectangular wood boards weighted with steel).  Most of this sampling takes place at night after ROV operations, but if the weather is too rough for the ROV, we will trawl during the day, sometimes in very deep water (1670 + m).  All of our tows are only 30 min, and this matches our data over the last 12 years, yielding a consistent data set for us to compare samples from the Gulf of Mexico to the Mid-Atlantic.  While the tows are short, the actual time to conduct deep-water tows can be long because the amount of wire we must let out is 2.5-3 times the water depth. For deep trawls we have out 4,000 m of wire; thus, one tow will require 5 hours of vessel time.

Kirstin Meyer sorts the catch from the trawl. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

Despite this long time commitment, trawling can be very productive and can give us data not available from other methods.  For example, this project has many objectives related to deep-sea corals.  Our last two trawl samples from water depths around 1,600 m yielded a number of solitary corals, and most of these came up alive.  These would have been hard to find with the ROV or other gear, and they will be used by our genetics team, the coral biologist, for our trophic objectives, and for distributional data.  The trawl also gave us several species of invertebrates and fishes not yet seen on the cruise.  These are used for several purposes (feeding studies, reproduction biology, ecology).  All data are useful and fit together to give us a better understanding of this poorly known ecosystem.

Science team members work up the catch from the trawl, including sorting, measuring and preserving the samples. Image courtesy of Liz Baird, Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS

While trawls produce a lot of our seafood, and as noted above can be valuable for science, they can also cause damage to bottom habitats.  We have seen lost trawl nets in these canyons wrapped around rocks and corals.  In addition, large commercial trawls catch a lot of animals that are not used, are discarded dead, and are thus wasted.  Fishermen do not want to lose their gear and many are very concerned about conservation.  Technological improvements (better bottom maps, better navigation, sensors on nets) are allowing trawling to become more efficient and less damaging; future improvements will continue to help these valuable fisheries.

The Museum received special greetings from the ROV Jason at the seep site during a presentation fed live into the Daily Planet theater. Image courtesy of Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS.

Spirits were high as we launched the connection to the Daily Planet at the North Carolina Museum of Natural Sciences on Wednesday. We had found the methane seep that we suspected to be in the area, and the video feed from the Jason ROV showed vast expanses of live mussels. Now we had a chance to share our passion for research with a new audience at the Museum.

Visitors in the Daily Planet participate in the interactive videoconference from the NOAA ship Ronald H. Brown. Image courtesy of Art Howard, ARTWORK.

Photographer Art Howard started the presentation at the Museum with images and video from some of our previous missions. He shared the stories we could not show live, such as the launch and recovery of the ROV, specimen processing and shipboard life. A participant in all of our deepwater canyons work, he’ll be bringing his artistic eye to the second leg of this mission. With the feed of the mussel fields behind us, Sandra Brooke, Brendan Roark and I answered questions posed by Meg Lowman, Director of the Nature Research Center. “What are some of the challenges of doing research at sea?” “Why are you studying the canyons?” “What are you learning about corals?” Midway through the discussion we interrupted the questions to point to the monitor. Written inside the lid of the bio-box on the Jason, 1600 meters down and with mussels as the backdrop, was a message: “Greetings to the NC museum of Natural Sciences.”   Live research coming to you not only from sea, but from the bottom of the deep sea!

BREAKING NEWS:

Mussels from the genus Bathymodiolus at the seep site found near Norfolk Canyon.
Image courtesy of Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS.

Tightly packed mussels formed mounds around the seep site. The rugged bottom appearance shows up on the sonar of the ROV Jason.
Image courtesy of Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS.

Push cores are used to collect samples of the sediment found near the seep site, allowing investigation into the biological, chemical and physical make up of the bottom.
Image courtesy of Deepwater Canyons 2013 Expedition, NOAA-OER/BOEM/USGS.

Perhaps the world’s largest methane cold seep area was discovered this week by an international, interagency team of scientists aboard the NOAA ship Ronald H. Brown, using the Woods Hole Oceanographic Institution’s ROV Jason II. This is only the third documented seep site on the US Atlantic Coast, and by far the most extensive; the two  paired seeps are estimated to be at least a kilometer long and in places hundreds of meters across. Densely packed seep mussels carpeted the bottom as far as the ROV cameras could see, and in a few locations large plumes of active methane bubbles streamed towards the surface from cracks in the seafloor. The seep mussels have bacteria living in their gills that use the methane to make energy and provide a constant energy source in the otherwise food-poor deep sea. This type of energy production is called chemosynthesis, and it allows large numbers of ‘chemosynthetic’ animals such as mussels to thrive over the seep area, along with a diverse community of other animals. Many of the mussels, as well as patches of the bottom, are covered by a thick white bacterial mat, which also thrives on the bubbling methane. Dead mussel shells on the periphery of the seep may indicate areas of past methane activity. At some seep sites there are tubeworms and clams that use sulfides instead of methane in a similar way. Unlike cold seeps found elsewhere, there were none of these seen at the new seep site, or the one explored last year in Baltimore Canyon. Sea cucumbers were seen tucked into the tight mounds of mussels and shrimp swam above them. Many species of fish, including some with unusual behaviors, were common around this unique ecosystem. These seeps are unique, and our time out here is just the beginning of the work it will take to begin to understand them. Co-led by Dr. Steve W. Ross of UNC-Wilmington and Dr. Sandra Brooke of Florida State University, the researchers from the numerous collaborating institutions used the diverse capabilities of Jason and the Ronald Brown to capture high definition video, sample the sediment at the site, collect live mussels for genetic and reproductive studies,  collect large dead shells and rocks for aging analysis, take water samples to examine water chemistry, as well as sample associated animals for examining food webs. Major funding for this expedition was provided to CSA Ocean Sciences and their collaborators by the Bureau of Ocean Energy Management, and NOAA provided funding for the Ronald H. Brown and Jason ROV.  US Geological Survey and other collaborators also provided a variety of resources.

Collaborating institutions and principal investigators include:

• Bureau of Ocean Energy Management
• National Oceanic and Atmospheric Administration
• CSA Ocean Sciences, Inc.
• Woods Hole Oceanographic Institution
• Univ. of North Carolina at Wilmington (Dr. S. W. Ross)
• Florida State University (Dr. S. Brooke)
• US Geological Survey (Drs. A. Demopoulos, C. Morrison, C. Kellogg, N. Prouty)
• Texas A&M University (Dr. B. Roark)
• Netherlands Institute of Sea Research (Drs. F. Mienis, G. Duineveld)
• Oregon Institute of Marine Biology (Dr. C. Young)
• University of Rhode Island (Dr. R. Mather)
• Univ. of Louisiana at Lafayette (Dr. S. France)
• Bangor University (Dr. A. Davies)
• ARTWORK, Inc. (A. Howard)
• NC Museum of Natural Sciences (L. Baird)